In vitro Regeneration of an Endangered Medicinal Orchid Dendrobium crepidatum Lindl. & Paxton through Protocorm like bodies.
نویسندگان
چکیده
A protocol for regeneration of an endangered medicinal orchid Dendrobium crepidatum was established mainly via indirect protocorm like body (PLBs) formation. The PLBs procured from in vitro growth cultures assessed ½ MS medium alone and combinations with 6-Benzyl amino purine (BAP), Kinetin (Kn) α-Naphthalene acetic acid (NAA). calli were induced longitudinally bisected segments PLBs. optimum percentage callus obtained supplemented BAP (1.0 mg/l) combination NAA (0.5 mg/l), where 64.3% 83.3% formed respectively. PLB formation achieved when transferred onto the respective basal medium. (91.6%) regenerated NAA. These differentiated into intact plantlets about 3 cm height 4-5 roots transplanted potting mixture highest rate 93.3% survival a green house.
منابع مشابه
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ژورنال
عنوان ژورنال: Plant Tissue Culture and Biotechnology
سال: 2023
ISSN: ['1818-8745', '1817-3721']
DOI: https://doi.org/10.3329/ptcb.v33i1.66753